生物知识 Subtelomeric and/or Subcentromeric Probe Sets Aside from microscopically visible chromosomal rearrangements, numerous cryptic ch... 05月05日 发表评论 阅读全文
生物知识 Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells Human embryonic stem cells (hESCs) are pluripotent cells derived from the embryo a... 05月05日 发表评论 阅读全文
生物知识 Methods for Homologous Recombination in Drosophila We present detailed protocols for two methods of gene targeting inDrosophila. The ... 05月05日 发表评论 阅读全文
生物知识 Introduction: Why Analyze Single Cells Powerful methods in molecular biology are abundant; however, in many fields includ... 05月05日 发表评论 阅读全文
生物知识 In Vitro and Ex Vivo Gene Delivery to Cells by Electroporation Electroporation generally refers to the technique of permeabilizing cell membranes... 05月05日 发表评论 阅读全文
生物知识 Detection of c-myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue by Chromogenic In In situ hybridization (ISH) allows evaluation of genetic abnormalities, such as ch... 05月05日 发表评论 阅读全文
生物知识 Polyamine Transport Systems in Mammalian Cells and Tissues Polyamine transport plays an important role in the homeostatic regulation of the p... 05月05日 发表评论 阅读全文
生物知识 DNA Fragmentation Assays for Apoptosis Protocol I:Triton X-100 Lysis BufferIn 96 flat-wells plate, incubate 4x10 6 target... 05月05日 发表评论 阅读全文
生物知识 Lanthanide-Based Upconversion Nanoparticles for Connexin-Targeted Imaging in Co-cultures From the perspective of deep tissue imaging, it is required that the excitation li... 05月05日 发表评论 阅读全文
生物知识 Digital Imaging of Stem Cells by Electron Microscopy This chapter deals with basic techniques of scanning and transmission electron mic... 05月05日 发表评论 阅读全文