生物知识 Methods for Assessing Autophagy and Autophagic Cell Death Autophagic (or type 2) cell death is characterized by the massive accumulation of ... 05月05日 发表评论 阅读全文
生物知识 Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria The biogenesis of mitochondrial membrane proteins is an intricate process that rel... 05月05日 发表评论 阅读全文
生物知识 HE 染色实验 简介包埋后的组织经过普通玻片切片后即可进行 HE 染色以达到形态学的观察需求,如果需要免疫组化或荧光分析,则切片时需要使用粘片剂处理玻片,防止多个操作步骤中的掉片。... 05月05日 发表评论 阅读全文
生物知识 Tracking Gene-Modified T Cells In Vivo Identification, monitoring, and analysis of genetically modified cells in the peri... 05月05日 发表评论 阅读全文
生物知识 Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers In tissue engineering, a variety of methods are commonly used to evaluate survival... 05月05日 发表评论 阅读全文
生物知识 Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles Purification of membrane proteins of necessity requires the use of detergents to s... 05月05日 发表评论 阅读全文
生物知识 Wound healing assay The wound healing assay allows the researcher to study cell migration and cell int... 05月05日 发表评论 阅读全文
生物知识 Quantification of Xenoprotein Electroinsertion in Mammalian Cells Recent studies reported the so-called “electroinsertion” of proteins having a memb... 05月05日 发表评论 阅读全文
生物知识 Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) comprise a diverse... 05月05日 发表评论 阅读全文
生物知识 Clonogenic Assays for Hematopoietic and Tumor Cells Using Agar-Containing Capillaries The so-called hematopoietic stem cells from mouse and human bone marrow can be ind... 05月05日 发表评论 阅读全文