1、A smear (rather than a single colony) of BAC colonies are picked and transferred...

1.Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl ,10 mM EDTA,50 mM...

Add NH4Ac (10 M stock or solid) to the sample for a final concentration of 2.5 M, ...

TA CloningI. Initial mixture5 μl dd H2O1 μl PCR product1 μl l...

1、Grow an overnight culture from a single colony in 2 mls LB antibiotic.2、Transfer...

IntroductionMethods and reagents is a unique monthly column that highlights curren...

Author: Long-Cheng LiSource: Protocol OnlineAbstract: Simplified method for prepar...

DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to 1...

ECKDescriptionThis method was suggested in a BRL "Focus" article several...

- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm secti...