The protocols listed here are for Drosophila cells in 6 well plates and our pre-al...
生物知识
生物知识
生物知识
In vitro transcription reaction
1 ml linearized DNA template pBS-SK- ERD (X-E) (0.1-0.5 mg)7.8 ml DDW5 ml 5X trans...
生物知识
RNAi术语表
RNA i GlossaryDicer - Dicer is a member of the RNase III family of nucleases that ...
生物知识
RNAi效果的测量
检测基因特异阻断的最常用的方法是进行westernblot分析，比较导入siRNA 前后蛋白表达水平的变化。在一些情况下可能使用检测报告子基因的报告子系统例如β-半...
生物知识
什么是RNA interference（RNAi）
RNA interference (RNAi ) by double stranded RNA (dsRNAs) molecules of approximatel...
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Background Information of RNAi
Background Information of RNA iRNA interference (RNA i) is a biological process in...
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The Easiest Route to Guaranteed
The Easiest Route to Guaranteed SilencingIncreasing numbers of researchers are usi...
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Total RNA extraction from Arabidopsis and tobacco–拟南芥和
RNA extraction buffer:0.1M NaCl2% SDS50mM Tris/HCl (pH9)10mM EDTA20mM ß-mercaptoet...
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Invitro transcription
The following protocol is for MEGAscript II Kit (Ambion).1. PCR amplify the DNA te...
生物知识
RNAi 实验介绍
1. RNAi 介绍 RNA 干扰（RNAi:RNA interference）是由诺贝尔生理学/医学奖得主 Andrew Z. Fire 和 Craig C. M...
