1. Pick single colony and inoculate 250 ml of LB broth containing 100 m g/l ampici...

ProcedureGrind in mortar and pestle or Waring blender with 5-7 volumes buffer A pe...

Materials:1.TENS solution:10 mM Tris (pH to 7.5)1 mM ethylenediaminetetraacetic ac...

1. Run gel (0.8 -1.0% agarose is best). For yeast chromosomal Southerns, digest 20...

The quality of sequencing results is directlyrelated to the quality of the templat...

This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from...

1. Remove 0.5 cm of tail into polypropylene microfuge tube (do not mince).(The tub...

Protocol for extracting DNA from ES Cells,starting from the 96-well plate but proc...

Protocol for Isolation of Genomic DNA from Mammalian Cells1.Trypsinize, harvest an...

This procedure was originally developed for Listeria monocytogenes but has worked ...