End-label oligo (20-25 mer)4 µl 5X T4 Kinase Buffer5 µl [gamma-32P] ATP (7000 Ci/m...
生物知识
生物知识
生物知识
Histochemical detection重组质体的检定
操作原理与colony hybridization 相同，但是将滤膜移置于含有IPTG 及5-bromo-4-chloro-3-indolyl β-D-glucur...
生物知识
Increasing Sensitivity in Northern Analysis with RNA Probes
Note: The pointers in this technical bulletin presuppose the use of standard 50% f...
生物知识
Genome Walking Kit 使用注意
用于Genome Walking Kit的模板总DNA 一般要满足以下几点：1、DNA 的完整性，电泳为单一条带。2、避免杂质，OD260/280=1.8~2.0。...
生物知识
Diatomaceous Earth-based Midi-prep
Diatomaceous Earth-based Midi-prep Note: This procedure is the method of choice fo...
生物知识
DNA extraction from blister rust aeciospores and urediniospores using dry grindi
Most recent version of protocol: 2/14/96Dr. Paul J. Zambino, Research Plant Molecu...
生物知识
DNA Plasmid Miniprep Protocol
1. Pick single colony and inoculate 5 ml of LB broth containing 200 g/l ampicillin...
生物知识
Purification of PCR fragments for cloning
After an aliquot of the PCR mixture is analyzed on an agarose gel, the remainder o...
生物知识
Concentration of DNA by ethanol precipitation
(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The Universi...
