一、mRNA的分离 与rRNA和tRNA不同的是，哺乳动物细胞的绝大部分mRNA在其3’端均有一poly(A)尾，因此可以用oligo(dT)－纤维素亲和层析法...
生物知识
生物知识
生物知识
C. elegans RNA prep
Introduction:C. elegans RNA preps that have been widely used previously involved f...
生物知识
PROBE PREPARATION(探针制备)
Please make sure to reference Andrew Hamilton for this protocolProbes : I use sing...
生物知识
Differential cDNA Screening Procedures（cDNA差异筛选）
The protocols listed refer to cDNA library construction and preliminary differenti...
生物知识
植物病毒RNA提取
大多植物病毒RNA为单链RNA，并且其极性与mRNA极性相同，植物病毒RNA提取较为简单，一般使用酚氯仿即可获得满意结果。 一、材料 提纯TMV病毒液（10...
生物知识
RNA AND PROTEIN EXTRACTION FROM THE SAME SAMPLE
This protocol was modified from the following referece: Bar-Peled, M., A.S. Concei...
生物知识
RNAse A Treatment of Mouse Cells
IntroductionRNAse A treatment of permeabilized cells followed by immunostaining is...
生物知识
用RNAi研究基因组印迹现象的调控机制
HHMI News-January 09,2004-New Insight into Control of Parental Gene Expression in ...
生物知识
植物病毒的RNA提取
大多植物病毒RNA为单链RNA，并且其极性与mRNA极性相同，植物病毒RNA提取较为简单，一般使用酚氯仿即可获得满意结果。 一、材料 提纯TMV病毒液（10mg/m...
生物知识
TRIzol Prep
Procedure 1. Homogenize cells (10 million) or tissue (50-100 mg) in 1 mL TRIzol Re...
