This protocol works well from a 5 ml starting culture or a 1 ml starting culture (...
生物知识
生物知识
生物知识
Cetyltrimethylammoniumbromide(CTAB)Pla
Procedure1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid ni...
生物知识
DNA isolation
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION F...
生物知识
Extraction of DNA from Schizophyllum commune （裂褶菌DNA提取）
1. Transfer plugs of mycelial margin to 0.1 P plates. Allow to grow to fill plate ...
生物知识
从琼脂糖和丙烯酰胺中纯化DNA
For fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller ...
生物知识
DNA的琼脂糖纯化(John Roth, Division of Biological Sciences,U
1.Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide.Lo...
生物知识
碱裂解法制备大肠杆菌质粒（Alkaline Lysis Plasmid Preparation）
This protocol yields plasmid DNA that is suitable for restriction digests and clon...
生物知识
Plasmid Mini-prep Protocol
1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on high...
生物知识
PREPARATION OF PLASMID DNA FOR SEQUENCING
The following protocol is based on our modifications of R. Kraft, J. Tardiff, K. S...
生物知识
Analys of Genomic DNA by Southern Hybridization
Localization of particular sequences within genomic DNA is usually accomplished by...
