Preparation of Cells1.Prepare or collect between 2x107 cells for each mRNA prep (w...
生物知识
生物知识
生物知识
Total RNA extraction from Arabidopsis and tobacco–拟南芥和烟草总RNA提取
RNA extraction buffer:0.1M NaCl2% SDS50mM Tris/HCl (pH9)10mM EDTA20mM ß-mercaptoet...
生物知识
植物RNA提取–Methods for Plant RNA Isolation
AMES/Chloroform extraction in our lab to extract total from potato leaves for viro...
生物知识
用一步法从细胞和组织中同时制备DNA、RNA和蛋白质
本文所述的是RNA提取的一步法的改进，可从少量组织和细胞中同时回收DNA、RNA和蛋白质。这种方法包括用含异硫氰酸胍和酚的一种单相液来裂解细胞，加入氯仿产生第二相，...
生物知识
变性RNA在膜上的转移和固定
多数情况下，为检测特定的靶mRNA，需先将RNA通过琼脂糖电泳分离后，再从胶上转移到二维支持物上（通常为尼龙膜），再与特异的标记探针杂交。本文主要介绍RNA在上行的...
生物知识
染色体的制片程序
1、取对数生长期细胞一个T75或T25瓶。2、将培养基换成10ml DMEM(H)+10%FBS+0.1ug/ml秋水仙素的培养基，处理1~2小时。3、将细胞培养液...
生物知识
Total RNA extraction from Arabidopsis and tobacco
RNA extraction buffer: 0.1M NaCl 2% SDS 50mM Tris/HCl (pH9) 10mM EDTA 20mM %26szli...
生物知识
Emobservations of microsomes
LEVEL I Figure 7.3 Isolated microsomes Figure 7.4 TEM of hepatocyteMATERIALS1% Glu...
生物知识
RNA purification — hot phenol protocol
Hot phenol may also be used to remove DNA.1. Add equal volume of TE-saturated phen...
生物知识
Phenol Extraction of rRNA
READ THROUGH ALL CAUTIONS BEFORE TRYING THIS EXPERIMENTMaterials•Rat liver (fasted...
