通常连接互相匹配的粘性末端可以产生新的酶切位点。以下组合是通过计算机程序计算出来的，尽管我们希望能尽量保证其准确性，但有些未经实验证实，因此不能保证100%的可行性...
生物知识
生物知识
生物知识
DNA rapid CTAB
A Rapid CTAB DNA Isolation TechniqueUseful for RAPD Fingerprinting and Other PCR A...
生物知识
FOOTPRINTING WITH DNASE1
1)probe: same as used for gelshift), isolated by isotacelectrophoresis w/out EtOH ...
生物知识
UV Shadowing
UV shadowing is a technique for visualizing nucleic acids separated on acrylamide/...
生物知识
Restriction Digestion of DNA
Restriction enzymes are expensive ($40 to $200 a vial): keep the enzymes at -20℃. ...
生物知识
NEB识别序列
下表中列出了NEB提供的所有限制性内切酶的特异性识别序列，同时列出的还有回文序列的每条互补链。比如CCTC(7/6) 和(6/7)GAGG均显示为MnlI识别序列。...
生物知识
EXO/S1 DELETION SERIES
1. Cut DNA with 5'- and 3'- overhangs, gel check, phenol-Sevag extract a...
生物知识
Partial Endonuclease Digestion
Prepare a 100 µl reaction mixture containing the DNA of interest in the appropriat...
生物知识
Thermal Inactivation
A simple, reversible way to a stop restriction reaction is by adding EDTA, which c...
生物知识
"Dirty" Mini Preps
1) Grow 3-5 ml over-night E. coli cultures containing your plasmid2) Spin down 1.5...
