Important : Extract the DNA within one week of receiving samples. Samples that hav...

1.grow up single colony in 1.5 ml LB/antibiotic ovn @37℃ .2.pour into tube, spin f...

Purpose: Purification of oligonucleotides (which have already been purified by rev...

1. Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide. ...

NOTE: THIS IS FINE FOR SOUTHERNS, BUT NOT FOR SCREENING BY PCR.(from Ruixia, 7/99,...

Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturated...

1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flasks, shake & pour int...

1. Pour a vertical acrylamide gel using TEA buffer. A 4 % non denaturing gel is co...

This is a standard large scale prep.for plasmid DNA which gives a yield of 0.5-1.0...

T-RFLP中文可翻译为：末端限制性片段长度多样性，又可以称为16sRNA基因的末端限制性片段分析技术。是一种新兴的研究微生物多态性的分子生物学方法，日亦受到研究人...