生物知识 PurificationofPCRfragmentsforcloning After an aliquot of the PCR mixture is analyzed on an agarose gel, the remainder o... 05月12日 发表评论 阅读全文
生物知识 ExtractionfromCheekCellsDNA Sample PreparationCheek cells are obtained by rinsing the mouth with 25mls of any ... 05月12日 发表评论 阅读全文
生物知识 Generation of uni-directionalnesteddeletion sin plasmid DNA 1) Digest DNA with two restriction enzymes such that one end is ExoIII susceptible... 05月12日 发表评论 阅读全文
生物知识 Dialysis (using dialysis tubing) Molecularpourous membrane tubing is used for desalting, protein and DNA isolation ... 05月12日 发表评论 阅读全文
生物知识 MITOCHONDRIALDNAISOLATION 1.Grind in mortar and pestle or Waring blender with 5-7 volumes buffer A per g tis... 05月12日 发表评论 阅读全文
生物知识 DNAPurificationandPrecipitation 1.Prepare or obtain Buffered phenol,pH 8.0.Add 2 crystals of 8-Hydroxyquinoline to... 05月12日 发表评论 阅读全文
生物知识 Quick DNA Plasmid Prep This protocol gives very clean plasmid preps for restriction digests and cloning. ... 05月12日 发表评论 阅读全文
生物知识 DNAPlasmidPrep. This is a scaled up version of the Baron protocol which has been modified to achie... 05月12日 发表评论 阅读全文
生物知识 Preparation of linear polyacrylamide Preparation of linear polyacrylamide used as carrier in ethanol precipitation of n... 05月12日 发表评论 阅读全文
生物知识 DNA Purification from Gels 1. Remove gel slice contain DNA fragment and place in 10 volumes of:300 mM NaOAc, ... 05月12日 发表评论 阅读全文