一、简介在制备新的病毒毒种贮液，或为蛋白质的生产和分析而进行感染时，了解毒种的滴度是非常重要的。二、操作方法虫媒病毒在鸡胚成纤维细胞中的空斑形成单位试验三、原理病毒...
生物知识
生物知识
生物知识
请教质谱高人啊，这结果怎么分析啊
我的蛋白样品到某公司去作简单质谱分析，利用的叫maldi－tof技术（激光解吸电离－飞行时间），可以分析样品的肽指纹图谱，他们还通过源后衰变（post souce ...
生物知识
紫外线诱变法
一、简介紫外线对微生物有诱变作用，主要引起是DNA的分子结构发生改变（同链DNA的相邻嘧啶间形成共价结合的胸腺嘧啶二聚体），从而引起菌体遗传性变异。将细胞计数后的平...
生物知识
膜印记固相杂交法（固相杂交-Southern 印迹杂交）
简介DNA的印迹杂交，又称Southern印迹杂交（Southern blotting），是指将通过凝胶电泳分离的DNA片段转移到特定的固相支持物上，在转移过程中D...
生物知识
Computer Simulation of MAPK Signal Transduction
We predict that computational modeling platforms will soon become standard tools i...
生物知识
Deoxyribozyme-Based, Semisynthetic Access to Stable Peptidyl-tRNAs Exemplified by tRNAVal Carrying a
We present a protocol for the reliable synthesis of non-hydrolyzable 3′-peptidyl-t...
生物知识
Microwave-Assisted Method for Fabrication of Carbohydrate Cluster Microarrays on 3-Dimensional Hydra
We present here a method for construction of carbohydrate cluster microarrays. Thi...
生物知识
Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli
We previously described the establishment of a binary vector system that allows co...
生物知识
Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect stimu
We propose two-dimensional gel electrophoresis (2-DE) and mass spectrometry to def...
生物知识
Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans
We provide information and protocols for the analysis of Rho GTPase function in ax...
