This chapter provides detailed descriptions of the protocol used for fish sperm cr...
生物知识
生物知识
生物知识
Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresis
This chapter reviews the CE analysis of bases, nucleosides, and single-stranded (o...
生物知识
Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis
This chapter will discuss various adaptations of the yeast two-hybrid method for a...
生物知识
Novel Eicosanoids: Isoprostanes and Related Compounds
This chapter will summarize the biochemistry and biological actions of isoprostane...
生物知识
50%终点法
原理50% 终点 (50% endpoint) 法是将病毒悬浮液经一系列稀释后，接种至动物、鸡胚或培养成单层的细胞，将每个稀释度造成的动物、鸡胚致死量或细胞病变做成...
生物知识
食品中常见的19种微生物污染、热处理和杀菌原理（二）
9、大肠埃希菌大肠埃希菌是人和动物肠道中的正常栖居菌。大肠埃希菌的致病物质之一是血浆凝固酶。根据致病性的不同，致泻性大肠埃希菌被分为产肠毒素性大肠埃希菌、肠道侵袭性...
生物知识
部分消化
原理有时需要得到仅在DNA片段的内部存在的部分限制性位点切割产生DNA，这在用待克隆片段内部存在的限制酶切位点进行克隆和构建酶切图谱时特别有用。材料与仪器DNA限制...
生物知识
基于化学诱变法产生随机突变体
简介化学诱变法（chemical mutagenesis）是将DNA 片段暴露于化学诱变剂如亚硝酸、羟胺、亚硫酸氢盐或阱，然后将 DNA 片段克隆形成一个小的文库，...
生物知识
Subtilisin-Catalyzed Glycopeptide Condensation
This method describes the use of subtilisin-catalyzed peptide condensation to form...
生物知识
Protein Staining Procedures
This method was successful in our lab using prostate tissue and for our specific o...
