This chapter describes a method for generation of the high-titer pseudotyped Molon...
生物知识
生物知识
生物知识
Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scatte
This chapter describes a method of sample preparation called “the rock and roll me...
生物知识
Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes
This chapter describes a nonradioactive method for the localization of mRNA in who...
生物知识
Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprintin
This chapter describes a number of aspects important for calibration of matrix-ass...
生物知识
On-Resin Convergent Synthesis of a Glycopeptide from HIV gp120 Containing a High Mannose Type N-Link
This chapter describes a rapid and efficient approach for the solid-phase synthesi...
生物知识
Cryopreservation of Avian Spermatozoa
This chapter describes a simple system for cryopreservation of avian spermatozoa a...
生物知识
相对 RT-PCR: 引物与竞争子比例的决定实验
材料与仪器双蒸水 RT-PCR 缓冲液 TaqDNA 聚合酶 cDNA dNTP 混合物 基因特异的正向引物 基因特异的反向引物 dCTPPCR 仪 PCR 管 Q...
生物知识
干墨水法
一、简介本实验介绍 3 种荚膜染色法：湿墨水法、干墨水法和Anthony 氏法。其中湿墨水法较简便、并适用于各种有荚膜的细菌。 二、操作方法 干墨水法三、原理荚膜是...
生物知识
浅析细菌不染色标本检查法
不染色标本一般用于观察细菌动力及运动情况，因为不染色标本直接在普通光学显微镜下，不能清楚看到细菌的形态与结构特征。细菌未染色时无色透明，在显微镜下主要靠细菌的折射率...
生物知识
酶联免疫斑点法（ELISpot）检测INF-γ
IFN-γ（干扰素-γ）主要产生于激活的T细胞和NK细胞。TH1型响应的特点是通过辅助T细胞和细胞毒T细胞产生IFN-γ，所以高水平IFN-γ的产生是由于寄主应对细...
