The principle of chiral ligand-exchange chromatography was introduced in the early...
生物知识
生物知识
生物知识
Characterization of RNase P RNA Activity
The principle task of the ubiquitous enzyme RNase P is the generation of mature tR...
生物知识
Assay of Retinol-Binding ProteinTransthyretin Interaction and Techniques to Identify Competing Ligan
The principles of fluorescence resonance energy transfer have been utilized to dev...
生物知识
菌落 PCR 实验
简介常规的 PCR 扩增需要进行细菌培养、质粒制备等多步操作后才能进行基因扩增，操作繁琐， 耗时较长，同时在反复的操作中 DNA 量损失也较大，产率较低。在 198...
生物知识
用新 RACE 法扩増 cDNA 的 5’末端
材料与仪器缓冲液、溶液和试剂 酶和酶缓冲液 核酸和寡核苷酸特殊设备步骤第 1 阶段：降解 RNA 的去磷酸化一、材料1. 缓冲液、溶液和试剂乙酸钠，3mol/L,p...
生物知识
羊膜腔接种
简介羊膜腔接种主要应用于从临床材料中（如患者咽嗽液等）分离流感病毒等操作。这种接种途径可直接感染羊膜腔的内胚层，也可被鸡胚咽下或吸入，引起全胚胎感染。另外，病毒也可...
生物知识
部分分解多肽两端有关的 PCR 法筛选 PK-120 基因实验基本方案
材料与仪器PK-120Sephadex-50 Gene Amp RNA PCR 试剂盒 合成寡聚核苷酸 抽提液 酵母 tRNAPCR 扩增仪步骤实验所需「试剂」具体...
生物知识
Hybridization and Detection of Digoxigenin Probes on RNA Blots
The probing of RNA gel blots (also called Northern blots) with labeled nucleic aci...
生物知识
In Vitro Transcriptions
The process of transcriptron in which a DNA template is used to derive RNA copies ...
生物知识
The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridomafusion: Immortalization of sensi...
