原理原位 PCR 利用了成熟发达的 PCR 或 RT-PCR 技术，使目的基因可以在固定组织或细胞的原位扩增；从而保证在亚细胞结构不被改变或破坏的情况下，允许目的基...
生物知识
生物知识
生物知识
ELISA检测乙脑病毒早期特异性IgM抗体
原理将抗μ链球蛋白（豚鼠抗兔IgM 抗体）非特异地吸附在固相上，加入待检乙脑病人血清，血清中的IgM 能和抗μ链球蛋白结合。当再加入定量特异性日本乙脑病（JBEV）...
生物知识
Transcription In Vitro Using Bacteriophage RNA Polymerases
Synthesis of specific RNA sequences in vitro is simplified because of the availabi...
生物知识
Integration of Phosphodiesterase-Induced Degradation of Oligonucleotides with Capillary Polymer-Siev
Synthetic oligodeoxyribonucleotides (ODN) have been proposed as a class of potenti...
生物知识
TAL Effector Nuclease (TALEN) Engineering
TALENs, fusion proteins of DNA binding domains of TAL (transcription activator-lik...
生物知识
Interpretation of Collision-Induced Fragmentation Tandem Mass Spectra of Posttranslationally Modifie
Tandem collision-induced dissociation (CID) mass spectrometry (MS) provides a sens...
生物知识
Tandem Mass Spectrometry in the Detection of Inborn Errors of Metabolism for Newborn Screening
Tandem mass spectrometry has been used for determinations of enzyme activities in ...
生物知识
Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute
Target validation for small RNAs in cells can be a confusing task wrought with pit...
生物知识
Probing Antibody-Antigen Interactions by Mass Spectrometry
Techniques that have been used for antigenic site mapping of linear epitopes in pr...
生物知识
Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
Telomerase is present in most human cancers, and proliferative stem cells includin...
