生物知识 慢病毒产生和滴度 简介根据所需的用途,sgRNA 库可以由慢病毒、逆转录病毒或腺相关病毒(AAV)提供。慢病毒和逆转录病毒整合到基因组中,而腺相关病毒不整合, 因此对于筛选,腺相关病... 04月18日 发表评论 阅读全文
生物知识 Using RNA Interference in Schistosoma mansoni Schistosomes are parasitic worms that infect over 200 million people and constitut... 04月18日 发表评论 阅读全文
生物知识 Dideoxyfingerprinting for Mutation Detection Screening for mutations of the thousands of the sequence products provided by huma... 04月18日 发表评论 阅读全文
生物知识 Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols Screening for mutations prior to sequencing can reduce the time and costs of ident... 04月18日 发表评论 阅读全文
生物知识 Gel-Forming and Cell-Associated Mucins: Preparation for Structural and Functional Studies Secreted and transmembrane mucins are important components of innate defence at th... 04月18日 发表评论 阅读全文
生物知识 Development of an On-Site Measurement System for Salivary Stress-Related Substances Based on Microch Secretory immunoglobulin A (sIgA) in saliva has been suggested to be a potential m... 04月18日 发表评论 阅读全文
生物知识 Oligo(dT)-纤维素层析分离Poly(A)+ RNA Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose ChromatographyJoseph Sambrook Pet... 04月18日 发表评论 阅读全文
生物知识 Covalent Coupling of DNA Oligonucleotides and Streptavidin Semisynthetic DNA-protein conjugates are synthesized by covalent coupling of thiol... 04月18日 发表评论 阅读全文
生物知识 Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry of Amino Acids, Peptides, and Pr Separation in capillary electrophoresis (CE) is based on the movement of charged c... 04月18日 发表评论 阅读全文
生物知识 HPLC Analytical Methods for the Separation of Molecular Species of Fatty Acids in Diacylglycerol and Separation of PLs or the lipid mediators DAG and PA into individual molecular spec... 04月18日 发表评论 阅读全文