Materials:3 M sodium acetate pH 5.2 or 5 M ammonium acetateDNA100% ethanolMeasure ...

(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The Universi...

FINGERPRINTING PROTOCOL (AGAROSE GEL) *Restriction digests consist of:15.75 ml ddH...

'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydro...

TE solution10 mM Tris (pH to 7.5)1 mM EDTA (pH to 8.0 to dissolve)2.Frozen agarose...

Used to removed unincorporated nucleotides from labelling reactions.Prepare Sephad...

Phenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies in ...

1、A smear (rather than a single colony) of BAC colonies are picked and transferred...

1.Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl ,10 mM EDTA,50 mM...

Add NH4Ac (10 M stock or solid) to the sample for a final concentration of 2.5 M, ...