PurposeDNA extraction without phenol extraction and centrifugation.Procedure1. Lys...

The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB with...

- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm secti...

Hi, does anyone know what is the best temperature and time for Isoprop precipitati...

1、Oligo design（1）The 5' end (the homology arm) - choose 42 or more (we usuall...

Phenol (removes protein)1.add equal volume of Phenol (= tris-saturated Phenol-Chlo...

DNA absorbs ultraviolet light due to its highly conjugated nature. DNA may thus be...

Day 11. Digest DNA for 6 hours (or overnight)BSA 10 mg/ml 0.5 22.65 ul of 10 ug Ge...

OverviewProcedure1. Preheat the CTAB Isolation Buffer at 60℃.2. Grind 2 g of fresh...

1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapilla...