1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on high...

1.Grow 5 ( large scale-15ml culture). Harvest in single eppendorf tube (or 15 ml d...

Phenol-based Method for the Isolation of DNA Fragments from Low-Melting Temperatur...

1. Prepare Whatman DE-52 according to manufacturers specifications. Equilibrate at...

I tried to clean a restriction product >10kb before ligation by a gel extractio...

Hattoti's protocol adapted to cell culture :Hattori M,Tugores A,Veloz L,Karin...

A protocol / method / schedule /procedure for extraction / isolation of both DNA a...

1.Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl ,10 mM EDTA,50 mM...

GEL PURIFICATION OF OLIGONUCLEOTIDES(Adapted from the method of Mark Fortini)1. Qu...