Use DeliverX Transfection from Panomics
Modified protocol below
Step 1: Make complexes
Note: Do not scale up more than 3x
Thaw siRNA and DeliverX reagent
Dilute siRNA into buffer 1
10 uM siRNA 2.17 ul
Buffer-1 47.8 ul
Sonicate DeliverX Reagent maximum output for 3-5 min
Dilute into buffer 2
DeliverX Reagent 16 ul
Buffer 2 34ul
Sonicate again for 3-5 min
Mix the diluted siRNA and the diluted DeliverX
Vortex
Incubate at 37 deg C for 20 min
Use immediately or store at -20 deg C for up to 6 months (<10 freeze/thaw)
Mix equal volumes buffer 1+ buffer 2
Add 300 ul mixed buffers to concentrated complexes (total 400 ul)
300
Step 2: Transfect cells
