Simultaneous analysis of DNA content and surface immunophenotype using gentle etha...
生物知识
生物知识
生物知识
层粘连蛋白(laminin, LN)
层粘连蛋白主要存在于基膜(basal lamina)结构中，是基膜所特有的非胶原糖蛋白, 相对分子质量为820kDa, 含13-15%的糖，有三个亚单位, 即重链(...
生物知识
Lentivirus的包装和感染
相关专题 1 转染前一天将293T细胞铺于10cm板，当细胞密度达到80％左右即可进行PEI转染。 2将下列DNA加入一个已装有480ul 1* HBS( PH 7...
生物知识
Generation of Antibodies That Are Externally Acting Isoform-Specific Inhibitors of Ion Channels
There is demand for isoform-specific ion channel inhibitors as tools to investigat...
生物知识
293细胞培养的经验总结
293细胞培养 293细胞是用5型腺病毒75株系转化，含有Ad5 E1区的人胚肾亚三倍体细胞系，是一种E1区缺陷互补细胞系。它是加拿大McMaster Univer...
生物知识
Hayflick界限(Hayflick life span)
1961年，Leonard Hayflick利用来自胚胎和成体的成纤维细胞进行体外培养，发现：胚胎的成纤维细胞分裂传代50次后开始衰退和死亡，相反，来自成年组织的成...
生物知识
生命科学中的FISH （“钓鱼”）技术
相关专题发展历史 荧光原位杂交 技术(fluorescence in situ Hybridization,FISH)问世于70年代后期,其曾多用于染色 体异常的研...
生物知识
Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos
The first step in elucidating the function of a particular gene of interest involv...
生物知识
Counting ES cell Chromosomes
(original reference: "tissue culture made easy" by Christian LaMantia fr...
生物知识
Detection of a Lipid-Lysine Adduct Family with an Amide Bond as the Linkage: Novel Markers for Lipid
An amide-type adduct, hexanoyl-lysine (HEL) is generated from the reaction between...
