相关专题 MAPK,丝裂原活化蛋白激酶(mitogen-activated protein kinases，MAPKs)是细胞内的一类丝氨酸/苏氨酸蛋白激酶。研究证...
生物知识
生物知识
生物知识
Monitoring Oncogenic B-RAF-Induced Senescence in Melanocytes
The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and...
生物知识
Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold
The study of neuronal connectivity requires the ability to trace axons from the ne...
生物知识
大鼠内皮细胞的培养方案
本人总结了下大鼠内皮细胞的培养方案 一，消化法。优点：获得的细胞比其他方法纯。缺点：获得细胞比较少。 常见问题及解决方法：1.消化的时间不好控制时，建议大家分两段时...
生物知识
Application of a Fusiogenic Peptide GALA for Intracellular Delivery
To enhance the cytosolic delivery of therapeutic drugs and genes, pH-sensitive and...
生物知识
ES and TS cell freezing/thawing
ES and TS cell freezing/thawingNeeded:ES cell freezing medium (2x)2x ES cell freez...
生物知识
Covalent Modification of G-Proteins by Affinity Labeling
The activation of heterotrimeric G-proteins is tightly regulated by the exchange o...
生物知识
Generation and Hepatic Differentiation of Human iPS Cells
A method for the generation of human induced pluripotent stem (iPS) cells was esta...
生物知识
Isolation of a Membrane-Bound Enzyme, 5-Nucleotidase
Membrane enzymes may be peripheral or integral proteins. The attachment of periphe...
生物知识
Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free C
This chapter describes the procedures in order to do immunofluorescence (IF) micro...
