生物知识 Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells The introduction of restriction endonucleases into mammalian cells in culture prov... 05月02日 发表评论 阅读全文
生物知识 Cryopreservation of Shoot-Tips and Meristems The ability to regenerate whole plants from cryopreserved, meristematic shoot tiss... 05月02日 发表评论 阅读全文
生物知识 Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells Head and neck reconstruction transplants often require a bony structure but also t... 05月02日 发表评论 阅读全文
生物知识 High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophi Preservation ofTetrahymena thermophilabasal body ultrastructure for visualization ... 05月02日 发表评论 阅读全文
生物知识 蛋白质寻靶(protein targeting) 游离核糖体合成的蛋白质在细胞内的定位是由前体蛋白本身具有的引导信号决定的。不同类型的引导信号可以引导蛋白质定位到特定的细胞器,如线粒体、叶绿体、细胞核和过氧化物酶体... 05月02日 发表评论 阅读全文
生物知识 Assessing PCP in the Cochlea of Mammalian Ciliopathy Models The increased availability of mouse models of human genetic ciliary diseases has l... 05月02日 发表评论 阅读全文
生物知识 Multiple Site-Directed Mutagenesis Site-directed mutagenesis techniques allow selective engineering of gene sequences... 05月02日 发表评论 阅读全文
生物知识 Serial Orthotopic Transplantation of Epithelial Tumors in Single-Cell Suspension Orthotopic transplantation of tumor tissue into recipient mice has long been estab... 05月02日 发表评论 阅读全文
生物知识 Strategies for the Development of Novel Taxol-Like Agents Taxol, the first microtubule stabilizer identified, is one of the most important n... 05月02日 发表评论 阅读全文