生物知识 Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. cri The glucosylation of 5-position of anthocyanin molecules is one of most important ... 05月03日 发表评论 阅读全文
生物知识 A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae Critical cellular processes such as DNA replication, DNA damage repair, and transc... 05月03日 发表评论 阅读全文
生物知识 Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity Cellular membranes, which contain abundant phospholipids, such as phosphatidylchol... 05月03日 发表评论 阅读全文
生物知识 Screening for Protein-Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells The two-hybrid system (THS) (1 ) is a molecular genetic screen that detects protei... 05月03日 发表评论 阅读全文
生物知识 Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi Genome sequences of many filamentous fungi are now available and additional genome... 05月03日 发表评论 阅读全文
生物知识 Control of the Interferon Response in RNAi Experiments The RNA interference (RNAi) and interferons have been an uneasy marriage. Ever sin... 05月03日 发表评论 阅读全文
生物知识 Overexpression or Downregulation of mTOR in Mammalian Cells This chapter presents an overview of the methods that have been used to overexpres... 05月03日 发表评论 阅读全文
生物知识 Competitive and Differential RT-PCR (CD-RT-PCR) for Measurement of Normalized Gene Expression Using Quantitative mRNA characterization by reverse transcription (RT) of RNA and subseq... 05月03日 发表评论 阅读全文
生物知识 Using Quantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Even The mechanisms that mediate how migratory eukaryotic cells amplify a shallow, extr... 05月03日 发表评论 阅读全文
生物知识 Biochemical Analysis of Arginine Methylation in Transcription Protein arginine methylation has emerged as an important mechanism for regulating ... 05月03日 发表评论 阅读全文