Zebrafish have emerged as a powerful model organism to study neutrophil chemotaxis...
生物知识
生物知识
生物知识
Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry
Isothermal titration calorimetry (ITC) is a useful technique to study RNA-protein ...
生物知识
Markers of Adult Neural Stem Cells
I provide detailed protocols for conduction and troubleshooting the key steps in o...
生物知识
siRNA表达载体的构建
siRNA表达载体构建可以：（1）作为后基因组时代基因功能分析的有力工具；（2）应用于基因组学、细胞信号传导通路分析；（3）用于药物靶点筛选、疾病治疗。实验方法原理...
生物知识
分子实验什么时候需要无菌操作？
当必须进行无菌操作时。无论什么时候在进行活的有机体实验或是为进行活体实验而准备培养基、缓冲液、培养容器等时，必须使用无菌操作技术，例如：·准备转化用的大肠杆菌培养物...
生物知识
常用的蛋白水解酶抑制剂
当细胞被裂解，内容物释放时，蛋白水解酶和其他一些降解的酶也一同被释放，这时候需要在裂解的缓冲液中加入蛋白水解酶。PS: 细胞自己的蛋白水解酶也会降解细胞内的蛋白质。...
生物知识
In Vitro Replication Assay with Mammalian Cell Extracts
Regulatory mechanisms for DNA replication are crucial to the control of the cell c...
生物知识
Tissue-Specific KO of ECM Proteins
The analysis of phenotypes caused by null and mutant alleles is a very powerful me...
生物知识
Isolation, Characterization, and Culture of Epithelial Stem Cells
It is well accepted that homeostasis of continuously renewing adult tissues, such ...
生物知识
Preparation of low density, collagenase-digested splenocytes Protocol
Preparation of low density, collagenase-digested splenocytes Protocol 1. Dilute 2 ...
