ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫学活性，酶标记的抗原或抗体既保留其免疫学活性，又保留酶的活性。在...
生物知识
生物知识
生物知识
Telomerase Inhibition and Telomere Targeting in Hematopoietic Cancer Cell Lines with Small Non-Nucle
Telomere maintenance has been shown to be essential for unlimited growth potential...
生物知识
Quantitative Determination of Collagen Crosslinks
The primary functional role of collagen is as a supporting tissue and it is now we...
生物知识
Purification of Nuclei and Preparation of Nuclear Envelopes from Skeletal Muscle
The nuclear envelope is a complex membrane-protein system that is notoriously diff...
生物知识
粘着连接(adherens junctions, zonula adherens)
在斑块连接中,如果连接作用涉及细胞质中的骨架部分是肌动蛋白，这种连接方式称为粘着连接。根据粘着连接涉及的是两个细胞间的连接还是细胞与细胞外基质的连接,又分为两种情况...
生物知识
Sequence Analysis of the Whole Mitochondrial Genome and Nuclear Genes Causing Mitochondrial Disorder
The diagnosis of mitochondrial disorders has increased considerably over the past ...
生物知识
Methods to Study Phosphorylation and Activation of the Hormone-Sensitive Adipocyte Phosphodiesterase
Cyclic nucleotide phosphodiesterases (PDEs) include a large group of structurally ...
生物知识
ChIP超详细实验步骤
ChIP的一般流程：甲醛处理细胞—收集细胞，超声破碎—加入目的蛋白的抗体，与靶蛋白-DNA复合物相互结合—加入ProteinA，结合抗体-靶蛋白-DNA复合物，并沉...
生物知识
胞质膜(cytoplasmic membrane)
存在于细胞质中各膜结合细胞器中的膜，包括核膜、内质网膜、高尔基体膜、溶酶体膜、线粒体膜、叶绿体膜、过氧化物酶体膜等。内容来源：生物资料网，如果侵权麻烦联系网站工作人...
生物知识
Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration
The function of many glycoproteins is directly related to the structure of their o...
