Flow cell assays are very useful for studying microtubule motility, microtubule dy...
生物知识
生物知识
生物知识
Isolation and Characterization of Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) have drawn great interest in the field of regenerati...
生物知识
Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents: Heterologous Expression
Voltage-sensitive Ca2+ channels (VSCC) play a central role in an extensive array o...
生物知识
Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells
We describe the immunostaining methods we commonly use to detect the more robust c...
生物知识
Proteomic Analysis of Scaffold Proteins in the ERK Cascade
ERK cascade scaffolds serve as docking platforms to coordinate the assembly of mul...
生物知识
Electrotaxis and Wound Healing: Experimental Methods to Study Electric Fields as a Directional Signa
Electric fields were measured at human skin wounds over one and half centuries ago...
生物知识
纤维切割蛋白(filament-severing protein)
这类蛋白能够同已经存在的肌动蛋白纤维结合并将它一分为二。由于这种蛋白能够控制肌动蛋白丝的长度，因此大大降低细胞中的粘度。经这类蛋白作用产生的新末端能够作为生长点, ...
生物知识
Methods for Culturing Human Embryonic Stem Cells in a Xeno-Free System
Defined pluripotent stem cell culture media is a valuable tool for tracking and an...
生物知识
侧向扩散(lateral diffusion)
又称侧向迁移。在同一单层内的脂分子经常互相换位, 其速度相当快, 有人推测磷脂以这种方式从细胞一端扩散到另一端只需1～2秒。这种运动始终保持脂分子在质膜中的排布方向...
生物知识
Bacterial Genome Sequencing
For over 30 yr, the Sanger method has been the standard for DNA sequencing. Instru...
