生物知识 Differentiate ES cells into glial cells and neurons Day -1: Pass ES cells at normal density on gelatinized plate to free the culture o... 05月04日 发表评论 阅读全文
生物知识 Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans A strategy for determining the structure of a glycosaminoglycan (GAG) can be divid... 05月04日 发表评论 阅读全文
生物知识 In Vivo and In Vitro Models of Mammalian Angiogenesis Angiogenesis is a complex process involving the organization of proliferating endo... 05月04日 发表评论 阅读全文
生物知识 Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies Mammary reconstitution assays can be used to measure the stem cell frequency withi... 05月04日 发表评论 阅读全文
生物知识 Analysis of Wnt Signaling During Caenorhabditis elegans Postembryonic Development Wnts play a central role in the development of many cells and tissue types in all ... 05月04日 发表评论 阅读全文
生物知识 Analysis of Myristoylated and Palmitoylated Src Family Proteins Several hundred viral and cellular proteins have been shown to be covalently modif... 05月04日 发表评论 阅读全文
生物知识 Generation and Establishment of Murine Adherent Cell Lines We describe a method to derive cell lines and clones from cells of the murine midg... 05月04日 发表评论 阅读全文
生物知识 细胞培养常见问题及回答 细胞培养常见问题及回答 如何选用特殊细胞系培养基? 培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞,很可能在DMEM或M199中同样很容易生长。总之,首选... 05月04日 发表评论 阅读全文
生物知识 核酶(ribozyme) 核酶一词用于描述具有催化活性的RNA, 即化学本质是核糖核酸(RNA), 却具有酶的催化功能。核酶的作用底物可以是不同的分子, 有些作用底物就是同一RNA分子中的某... 05月04日 发表评论 阅读全文
生物知识 Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identifica The nuclear envelope (NE) is a double membrane system that is both a part of the e... 05月04日 发表评论 阅读全文