It is now clear that the adult central nervous system contains a population of neu...
生物知识
生物知识
生物知识
Trypsinizing cells
There are many procedures with which to trypsinize cells. All include washing the ...
生物知识
Cryopreservation of Semen from Domestic Livestock
Although the first observations concerning low temperature preservation of spermat...
生物知识
细胞冻存方法
1．细胞：选对数生长期细胞，收集细胞24小时前换液一次。2．计数：按常规方法把细胞制成细胞悬液，计数，令细胞密度达5×106/ml，离心去上清，吸入离心管中。3．冻...
生物知识
细胞增殖的检验方法：BrdU标记法
BrdU标记法1． 细胞以 1.5 × 105 /ml 细胞数接种于直径 35ml 培养皿中（内放置一盖玻片），培养 1 天，用含 0.4 ％ FCS 培养液同步化...
生物知识
Assays for the Evaluation of HIV-1 Integrase Inhibitors
Integration is an essential step in the replication cycle of a retrovirus, such as...
生物知识
Expression and Refolding of Full-Length HumanTIMP-1
Structural analysis of any protein, whether by X-ray crystallography or NMR, requi...
生物知识
Live Microscopy of Neural Stem Cell Migration in Brain Slices
In the developing central nervous system (CNS), neural stem cells undergo a comple...
生物知识
Detection of Apoptosis by TUNEL Assay
Terminal deoxynucleotidyl transferase (TdT) dUTPNick-EndLabeling (TUNEL) assay has...
生物知识
踏车现象(treadmilling)
又称轮回,是微管组装后处于动态平衡的一种现象。微管的两端都可以加上αβ二聚体, 或释放αβ二聚体。但在" "端, 由于结合有GTP帽结构的存在,...
