生物知识 Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays The ribonuclease protection assay (RPA) is a sensitive and straightforward method ... 05月05日 发表评论 阅读全文
生物知识 Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes The purification of native protein complexes requires the availability of sufficie... 05月05日 发表评论 阅读全文
生物知识 Experimental Models to Analyze Differentiation Functions of Cultured Keratinocytes In Vitro and In V In this chapter, we present technical details for the generation of in vitro skin ... 05月05日 发表评论 阅读全文
生物知识 Analysis of the Fission Yeast Schizosaccharomyces pombe Cell Cycle Fission yeast is a popular model organism for the study of the cell cycle. It grow... 05月05日 发表评论 阅读全文
生物知识 细胞学技术的一般环节简介 相关专题 一、纺锤体阻断剂(Spindle inhibitor) 在有丝分裂过程中,随着纺锤丝的形成,染色体 被牵引到一起难以观察其形态。纺锤体的形成在于细胞质和纺... 05月05日 发表评论 阅读全文
生物知识 肿瘤发生(tumorigenesis) 正常细胞发展成为肿瘤的过程称为肿瘤发生。从细胞水平上看,癌的发生是极偶然的事件。从遗传上看,癌都是由一个细胞发展而来,由一个失去了增殖控制的细胞发展而来。人体有百万... 05月05日 发表评论 阅读全文
生物知识 Isolation and Characterization of Mouse Side Population Cells The side population (SP) is a subpopulation of mouse bone marrow cells highly enri... 05月05日 发表评论 阅读全文
生物知识 Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosectio MicroRNAs (miRNAs) are 20−25 nucleotide long, noncoding, and single-strand RNAs th... 05月05日 发表评论 阅读全文
生物知识 Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells Although embryonic stem (ES) cells have the regenerative capability of producing a... 05月05日 发表评论 阅读全文
生物知识 Superfused Microcarrier Cultures of BeWo Choriocarcinoma Cells: A Dynamic Model for Studies of Human The human placenta is a unique and still very poorly understood organ During gesta... 05月05日 发表评论 阅读全文