Gene therapy provides a paradigm of the treatment of human diseases. The ultimate ...

Integrins play critical adhesion and signaling roles during development, wound hea...

Recycling TubulinWe "recycle" tubulin fractions stored at -80¡C after th...

TheXenopus laevisoocyte offers one of the most convenient expression systems for a...

This chapter is a step-by-step protocol for setting up, realizing, and analyzing s...

Aside from microscopically visible chromosomal rearrangements, numerous cryptic ch...

Human embryonic stem cells (hESCs) are pluripotent cells derived from the embryo a...

We present detailed protocols for two methods of gene targeting inDrosophila. The ...

Powerful methods in molecular biology are abundant; however, in many fields includ...

Electroporation generally refers to the technique of permeabilizing cell membranes...