The yeast two-hybrid system is a powerful tool for the isolation and characterizat...
生物知识
生物知识
生物知识
Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as P
The basic principle for probing the transverse distribution of membrane lipids is ...
生物知识
全能性(totipotency)
分化细胞保留着全部的核基因组, 它具有生物个体生长、发育所需要的全部遗传信息, 即能够表达本身基因库中的任何一种基因, 也就是说分化细胞具有发育为完整个体的潜能, ...
生物知识
Methods for Studying the G2 DNA Damage Checkpoint in Mammalian Cells
In response to post-replicative DNA damage, cells activate the G2 DNA damage check...
生物知识
High-Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant
Intracellular Ca2+ mobilization is a useful readout to screen for agonists or anta...
生物知识
Apoptosis: A Laboratory Manual of Experimental Methods
HE CELL1. Morphological aspects of apoptosisWalter Malorni, Stefano Fais & Car...
生物知识
Perforated Whole-Cell Patch-Clamp Recording
Perforated whole-cell patch-clamp is a variant of the patch-clamp technique used t...
生物知识
常规组织培养法
相关专题 一、初代消化培养法 1. 准备：取各种已消毒的培养用品置于净化台面，紫外线消毒20分钟。开始工作前先洗手、75%酒精擦拭手至肘部。 2.布局：点燃酒精灯，...
生物知识
终变期(diakinesis)
前期I的最后一个阶段，又称再凝集期(recondensation stage)。此期染色质又被包装压缩成染色体。大多数核仁消失，四分体均匀地分布在核中。染色体交叉逐...
生物知识
Measuring Protein Kinase and Sugar Kinase Activity in Plant Pathogenic Fusarium Species
As ubiquitous metabolic and signaling intermediaries, kinases regulate innumerable...
