简介肠吻合术是指小肠切断后，两断端可用小肠-小肠吻合术进行吻合，以恢复肠袢的连续性。材料与仪器器材：血管钳、纱布、肠钳、有齿血管钳试剂：①生理盐水②0.5% 氯已定...
生物知识
生物知识
生物知识
动物实验外科手术吻合术-神经吻合术
简介神经吻合术，主要是将断裂的神经进行吻合，恢复正常的神经功能。材料与仪器器材：丝线或尼龙线步骤神经吻合术的基本过程可分为如下几步：A. 将两断端稍加修剪整齐，尽量...
生物知识
气管插管术
简介气管插管术在许多动物手术中为了保持动物呼吸道通畅，减少病死率进行的实验。用途气管插管术可用于大动物如犬、猪等，气管插管操作较容易，咄齿类动物操作困难，常行气管切...
生物知识
血管插管术
简介血管插管术是动物实验中常常使用的操作手术，通常是由于手术中需要反复采血、给药、补液等。血管插管最常选用的血管有颈外静脉、颈总动脉、股静脉、股动脉等，这些血管分布...
生物知识
Tracking Gene-Modified T Cells In Vivo
Identification, monitoring, and analysis of genetically modified cells in the peri...
生物知识
Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers
In tissue engineering, a variety of methods are commonly used to evaluate survival...
生物知识
Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles
Purification of membrane proteins of necessity requires the use of detergents to s...
生物知识
Wound healing assay
The wound healing assay allows the researcher to study cell migration and cell int...
生物知识
Quantification of Xenoprotein Electroinsertion in Mammalian Cells
Recent studies reported the so-called “electroinsertion” of proteins having a memb...
生物知识
Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP
G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) comprise a diverse...
