生物知识 程序性细胞死亡(programmed cell death,PCD) 又称为细胞凋亡(apoptosis)。程序性细胞死亡是指为维持内环境稳定,由基因控制的细胞自主的有序性的死亡,它涉及一系列基因的激活、表达以及调控等的作用,因而是具... 05月05日 发表评论 阅读全文
生物知识 Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromato The epidermal growth factor (EGF) receptor possesses intrinsic protein-tyrosine ki... 05月05日 发表评论 阅读全文
生物知识 Use of Multiphoton Imaging for Studying Cell Migration in the Mouse We describe a method for studying cell motility in the living mouse using multipho... 05月05日 发表评论 阅读全文
生物知识 Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates T his procedure allows detection of specific cell adhesion to glycolipids resolved... 05月05日 发表评论 阅读全文
生物知识 Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration This protocol describes an in vivo assay for random and directed hemocyte migratio... 05月05日 发表评论 阅读全文
生物知识 The Application of X-Ray Absorption Spectroscopy to Characterize Metal Centers in Proteins The availability of synchrotron radiation has transformed the status of X-ray abso... 05月05日 发表评论 阅读全文
生物知识 Isolation and Characterization of Human Embryonic Stem Cells The derivation of human embryonic stem (hES) cells is a challenging procedure. The... 05月05日 发表评论 阅读全文
生物知识 Cultivation of Normal Human Epidermal Melanocytes An important approach in studies of normal, diseased, and malignant cells is their... 05月05日 发表评论 阅读全文
生物知识 Methods for Assessing Autophagy and Autophagic Cell Death Autophagic (or type 2) cell death is characterized by the massive accumulation of ... 05月05日 发表评论 阅读全文
生物知识 Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria The biogenesis of mitochondrial membrane proteins is an intricate process that rel... 05月05日 发表评论 阅读全文