生物知识 Specialized Techniques for Site-Directed Mutagenesis in Cyanobacteria Synechococcus elongatusPCC 7942 is an excellent model system for studying the mole... 05月05日 发表评论 阅读全文
生物知识 Structural Studies on MMPs andTIMPs Wolfram Bode and Klaus Maskos The proteolytic activity of the matrix metalloproteinases (MMPs) involved in extra... 05月05日 发表评论 阅读全文
生物知识 Scanning Electron Microscopy of Bone This chapter described methods for Scanning Electron Microscopical imaging of bone... 05月05日 发表评论 阅读全文
生物知识 Selective Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage The targeted delivery of fluorescently labeled, DNA-modifying proteins into cellul... 05月05日 发表评论 阅读全文
生物知识 Small-Angle X-Ray Scattering Studies of Membrane Transporter Peripheral Components and Soluble Domai Structural data are critical for an understanding of how membrane transport system... 05月05日 发表评论 阅读全文
生物知识 In Vivo Labeling and Analysis of Epidermal Stem Cells To identify slowly cycling (label-retaining) keratinocytes in the cutaneous epithe... 05月05日 发表评论 阅读全文
生物知识 我对付支原体污染的一点经验 前阵子做单抗,做了几次细胞融合都没做出来,后面怀疑是SP2/0受支原体污染,细胞生长速度减慢,出现细胞碎片,换液后情况稍好。怀疑受污染后我对细胞做了一系列的处理,终... 05月05日 发表评论 阅读全文
生物知识 Functional Analysis by Inducible RNA Interference in Drosophila melanogaster Ribonucleic acid (RNA) interference triggered by double-stranded RNA has become a ... 05月05日 发表评论 阅读全文
生物知识 Expression of I-Sce I in Drosophila to Induce DNA Double-Strand Breaks Generation of double-strand breaks (DSBs) in chromosomal DNA induces repair machin... 05月05日 发表评论 阅读全文
生物知识 Mitophagy and Mitoptosis in Disease Processes Mitochondria play a very important role in cellular function, not only through key... 05月05日 发表评论 阅读全文