一、从土壤中分离放线菌1.制作高氏一号培养基，趁热注入培养皿中，凝成平板，待用。2.称取土壤10克，放入装有100毫升无菌水的锥形瓶中，并加入10%酚10滴，以抑制...
生物知识
生物知识
生物知识
反向 PCR
原理标准 PCR 用来扩增位于两条引物内侧的 DNA 片段，与此相反，反向 PCR 技术（也被称为倒置或者向外 PCR）是用于扩增一段已知 DNA 序列旁侧的 DN...
生物知识
油脂水解试验
一、简介微生物对大分子的淀粉、蛋白质和脂肪不能直接利用，必须靠产生的胞外酶将大分子物质分解才能被微生物吸收利用。胞外酶主要为水解酶， 通过加水裂解大的物质为较小的化...
生物知识
Neurophenotyping Genetically Modified Mice for Social Behavior
Sociability in mice is a multidimensional adaptive and functional response. Due to...
生物知识
Western Immunoblot Analysis
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) ( 1 ) combine...
生物知识
Crystallographic Analyses of Chemically Synthesized Modified Hammerhead RNA Sequences as a General A
Solid-support chemical synthesis of RNA (1 ), though costly, has the advantage of ...
生物知识
Solubilization and Assay of Cellular and Tissue Protein
Solubilization and accurate quantification of cellular protein are central to anal...
生物知识
Separation and Size Determination of DNA over a 10,200 kbp Range
Some types of pulsed-field gel electrophoresis (PFGE), including CHEF (contour-cla...
生物知识
The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM) Purging and Hematopoietic Progenitor Cell E
Soybean agglutinin (SBA) is a plant lectin, a glycoprotein of nonimmune origin tha...
生物知识
Crosslinking of Single-Stranded DNA to Resins
Specific fragments of single-stranded DNA may be covalently immobilized to solid s...
