生物知识 Serglycin: The Master of the Mast Cell Serglycin is a proteoglycan composed of a relatively small (∼17 kDa) core protein ... 04月18日 发表评论 阅读全文
生物知识 Preparation and Assay of Recombinant Serine Racemase Serine racemase is a glial and neuronal enzyme that reversibly converts l -serine ... 04月18日 发表评论 阅读全文
生物知识 CE Analysis of -Globulin Mobility and Potential Clinical Utility Serum protein electrophoresis is widely used in clinical laboratories to measure t... 04月18日 发表评论 阅读全文
生物知识 Nonreducing 2-D Polyacrylamide Gel Electrophoresis Several approaches can be used for identifying 2-DE resolved proteins, including m... 04月18日 发表评论 阅读全文
生物知识 半巢式 PCR 简介半巢式 PCR 是利用三条引物进行两次 PCR 扩增的方法,与巢式 PCR 原理相同,只是在第 2 轮 PCR 反应中使用的引物有 1 条为第 1 轮 PCR ... 04月18日 发表评论 阅读全文
生物知识 High-Resolution, 2-D Protein Electrophoresis Using Nondedicated Equipment Several formats—small, standard, and large—can be employed to separate proteins by... 04月18日 发表评论 阅读全文
生物知识 Ubiquitin Chain Synthesis Several important signaling processes depend on the tagging of cellular proteins w... 04月18日 发表评论 阅读全文
生物知识 Formation and Characterization of Hepatocyte Spheroids Several investigators have demonstrated that freshly harvested hepatocytes self-as... 04月18日 发表评论 阅读全文
生物知识 Dealing with different methods for Kluyveromyces lactis -galactosidase purification Several micro-scale chromatography-based procedures for purification of the β-gala... 04月18日 发表评论 阅读全文
生物知识 Use of three-dimensional collagen gels to study mechanotransduction in t47d breast epithelial cells Several pathological and disease conditions can alter the mechanical properties of... 04月18日 发表评论 阅读全文