一、简介制备好感受态细胞后，接下来就是质粒DNA转化入大肠杆菌细胞的过程。但要注意的是感受态细胞最好是新制备的，因为保存一定时间的感受态细胞会使转化率降低；此外DN...
生物知识
生物知识
生物知识
Nanoporous Silica Glass for the Immobilization of Interactive Enzyme Systems
Recent pursuit on utilization of nanoscale materials has manifested a variety of c...
生物知识
Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation
Recent studies have revealed that rabbit reticulocyte lysate (RRL) efficiently rec...
生物知识
A Brief Summary of the Different Types of Mass Spectrometers Used in Proteomics
Recent technical innovations in mass spectrometry-based techniques have resulted i...
生物知识
Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry for Protein and Peptide Profiling of
Recently, Ciphergen Biosystems (Fremont, CA, USA) has developed a technique called...
生物知识
Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphoryl
Recently, it has been shown that enzymes of the cyclic adenosine monophosphate (cA...
生物知识
Analysis of Triplet-Repeat DNA by Capillary Electrophoresis
Recently, much attention has been focused on triplet-repeat expansions on the huma...
生物知识
Site-Specific Immobilization of Antibodies to Protein G-Derivatized Solid Supports
Recombinant DNA-based manufacturing of biologicals has necessitated the developmen...
生物知识
A method for rapidly screening functionality of actin mutants and tagged actins
Recombinant production and biochemical analysis of actin mutants has been hampered...
生物知识
Retroviral-mediated gene transfer in primary murine and human T-lymphocytes
Recombinant retroviruses are efficient vectors for introducing genes into many mam...
