Reactive oxygen species are implicated in many human diseases and aging process. M...
生物知识
生物知识
生物知识
Electron Paramagnetic Resonance Oximetry and Redoximetry
Reactive oxygen/nitrogen species (ROS/RNS) have been increasingly recognized as im...
生物知识
Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phos
Reactive-oxygen species and organic free radicals are essential metabolic intermed...
生物知识
WB 过程中常见问题及建议大汇总
Western 免疫印迹（Western Blot）是将蛋白质转移到膜上，然后利用抗体进行检测。对已知表达蛋白，可用相应抗体作为一抗进行检测，对新基因的表达产物，可...
生物知识
赛默飞PCR常见问题
PCR产物的电泳检测时间 一般为48h以内，有些最好于当日电泳检测，大于48h后带型不规则甚致消失。假阴性，不出现扩增条带 PCR反应的关键环节有①模板核酸的制...
生物知识
Protein A Purification of Antibody
Reagents Affi-gel Protein-A Agarose (BioRad #153-6153) MAPS II Binding Buffer (Bio...
生物知识
DGK Membrane Preparation
Reagents: Bacterial strainE.coli N4830/pJW10 LB amp media 50 µg/ml ampicillin High...
生物知识
Phosphate Assay by Suprya Jaydev
ReagentsAshing buffer:10 g Mg(NO3)2 100 ml EtOH1.5 N HCl stock:119.7 ml concentrat...
生物知识
Indirect Immunofluorescence
Reagents: NGS-Normal Goat Serum PBST-0.5% Tween-20/CMF-PBS CMF-PBS 1°Ab-mouse MF20...
生物知识
In vitro Sphingomyelinase Assay
Reagents:Lysis buffer 25 mM Tris-HCl, pH 7.4 5 mM EDTA 1 mM ATP 20 µg/ml CLAP 1 mM...
