生物知识 Absolute Quantitation of 2-D Protein Spots Proteome analysis has been revolutionized by the marriage of two-dimensional (2-D)... 04月18日 发表评论 阅读全文
生物知识 Identification of Components of Protein Complexes Protocols are given for a variety of techniques used in protein identification of ... 04月18日 发表评论 阅读全文
生物知识 利用高温PCR体系改善高(G+C)含量DNA模板的PCR扩增 简介DNA 模板形成的二级和三级结构,在高温环境中呈现变性或不完全变性状态。为了不使变性的 DNA 在低温下再次恢复复杂结构,将整个 PCR 扩增过程包括变性、退火... 04月18日 发表评论 阅读全文
生物知识 Site-Directed Mutagenesis with LA-PCR Technology Protocols for site-directed mutagenesis are widely used in molecular biology and i... 04月18日 发表评论 阅读全文
生物知识 Genotyping for Cytochrome P450 Polymorphisms Protocols for the extraction of DNA from human blood and for genotyping for a numb... 04月18日 发表评论 阅读全文
生物知识 Protozoan Genomes: Karyotype Analysis, Chromosome Structure, and Chromosome Specific Libraries Protozoa represent a diverse group of single-celled eukaryotes, many of which have... 04月18日 发表评论 阅读全文
生物知识 Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA Pulsed-field gel electrophoresis (PFGE) has been successfully used to generate phy... 04月18日 发表评论 阅读全文
生物知识 Theories of Pulsed-Field Gel Electrophoresis Pulsed-field gel electrophoresis (PFGE) is one of the key technological advances o... 04月18日 发表评论 阅读全文
生物知识 Purification and cloning of DNA fragments fractionated on agarose gels Purification of DNA fragments from acrylamide or agarose gels is a commonly used t... 04月18日 发表评论 阅读全文
生物知识 The influence of photoperiodic growth condition on isolation of RNA from strawberry (Fragaria ananas Purification of high-quality RNA from different strawberry tissues is often affect... 04月18日 发表评论 阅读全文