This is a four day procedure so it's best to start on Monday or Tuesday.CASE ...

ReagentsChloroform EDTA,0.5 M Ethanol, absolute Isoamyl alcohol Sigma,Cat.I-3643 P...

1.grow plants in trays of 96 and leave two spots open (for the PCR controls)2.harv...

1、A smear (rather than a single colony) of BAC colonies are picked and transferred...

macerate tissue in Eppendorf tube without butter at RTadd 400 m l extraction buffe...

PrincipleThe nucleic acids contained within the gel slice are electrophoresed out ...

Author: Suzanne GerttulaDate: March 14,1994Digest between 1 and 10 ug Genomic DNA....

PEG Preparation of PlasmidPlasmid isolated by this procedure can be used routinely...

This is a scaled up version of the Baron protocol which has been modified to achie...

End-label oligo (20-25 mer)4 µl 5X T4 Kinase Buffer5 µl [gamma-32P] ATP (7000 Ci/m...