Mitogen-activated protein kinase (MAPK) signaling influences a variety of cellular responses, ranging from stimulation of cell proliferation to induction of senescence and/or apoptosis. Ca2+ is a ubiquitous intracellular signaling molecule that controls multiple processes in cells. Published evidence has identified both direct and indirect interactions between the Ca2+ and MAPK signaling pathways. Here, we describe assays to accurately determine the effect of changes in intracellular Ca2+ concentration on MAPK activation.
