Cleavage Sites Table for Fusion Proteins:
PROTEASE | COMPANY | Protease Capture |
ThrombinLeu-Val-Pro-Arg�Gly-Ser | Amersham-Biosciences, Novagen,SIGMA, Roche | Benzamidine-Agarose |
Factor XaIle-Glu/Asp-Gly-Arg� | Amersham-Biosciences, New England Biolabs,Roche | Benzamidine-Agarose |
EnterokinaseAsp-Asp-Asp-Asp-Lys� | New England Biolabs,Novagen, Roche | Trypsin Inhibitor-Agarose |
TEV proteaseGlu-Asn-Leu-Tyr-Phe-Gln�Gly | Invitrogen – Life Technologies | Ni-NTA (6His recomb. TEV) |
PreScissionLeu-Glu-Val-Leu-Phe-Gln�Gly-Pro | Amersham-Biosciences | GSTrap for GST fusion enzyme |
TAGZymeHis-tag removal by Exoproteolytic Digestion | Qiagen | Ni-NTA (6His recomb. enzyme) |
Intein Sitedithiothreitol cleavage | New England Biolabs | DTT elimination by dialysis |
HRV 3C ProteaseLeu-Glu-Val-Leu-Phe-Gln�Gly-Pro | Novagen | Ni-NTA (6His recomb. enzyme) |
SUMO Proteaserecognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO | Invitrogen – Life Technologies, LifeSensors | Ni-NTA (6His recomb. enzyme) |
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