切除融合标签常用蛋白酶的酶切位点

Cleavage Sites Table for Fusion Proteins:

PROTEASE COMPANY Protease Capture
ThrombinLeu-Val-Pro-Arg�Gly-Ser Amersham-Biosciences, Novagen,SIGMA, Roche Benzamidine-Agarose
Factor XaIle-Glu/Asp-Gly-Arg� Amersham-Biosciences, New England Biolabs,Roche Benzamidine-Agarose
EnterokinaseAsp-Asp-Asp-Asp-Lys� New England Biolabs,Novagen, Roche Trypsin Inhibitor-Agarose
TEV proteaseGlu-Asn-Leu-Tyr-Phe-Gln�Gly Invitrogen – Life Technologies Ni-NTA (6His recomb. TEV)
PreScissionLeu-Glu-Val-Leu-Phe-Gln�Gly-Pro Amersham-Biosciences GSTrap for GST fusion enzyme
TAGZymeHis-tag removal by Exoproteolytic Digestion Qiagen Ni-NTA (6His recomb. enzyme)
Intein Sitedithiothreitol cleavage New England Biolabs DTT elimination by dialysis
HRV 3C ProteaseLeu-Glu-Val-Leu-Phe-Gln�Gly-Pro Novagen Ni-NTA (6His recomb. enzyme)
SUMO Proteaserecognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO Invitrogen – Life Technologies, LifeSensors Ni-NTA (6His recomb. enzyme)

内容来源:生物资料网,如果侵权麻烦联系网站工作人员删除!

艾美捷科技优势代理品牌

发表评论

:?: :razz: :sad: :evil: :!: :smile: :oops: :grin: :eek: :shock: :???: :cool: :lol: :mad: :twisted: :roll: :wink: :idea: :arrow: :neutral: :cry: :mrgreen: