A variety of probes can be used forin situhybridization, depending on the application and the labeling strategy. In general, RNA probes (riboprobes) are now more commonly used. However, some laboratories may still prefer to use DNA probes forin situhybridization, and a number of techniques for this type of labeling have been described in this chapter. The preparation of plasmid DNA and the subsequent use of polymerase chain reaction products as labeling templates are discussed in detail. Both radioactive and nonradioactive labeling procedures are described and the latest nonradioactive detection methods are outlined.
