Recommended Reaction Conditions: Initial Conditions:
Initial denaturation at start:92 - 97oC for 3 - 5 min. If you denature at 97oC, denature sample only; add rest of mix after reaction colls to annealing temperature (prevents premature denaturation of enzyme).
Initial annealing temperature:as high as feasible for 3 min (eg: 50 - 75oC). Stringent initial conditions mean less non-specific product, especially when amplifying from eukaryotic genomic DNA.
Initial elongation temperature:72oC for 3 - 5 min. This allows complete elongation of product on rare templates.
PCR方法相关产品:
- 电泳设备
- 紫外设备
- 普通PCR仪
- 定量PCR仪
- PCR/RT-PCR/qPCR试剂
- PCR引物
- PCR试剂
- PCR对照
- 特异性PCR试剂盒
- PCR克隆试剂盒
- RNA
- RNase检测/去除
- RT-PCR试剂
- RT-PCR标准品
- 定量PCR试剂
- 定量PCR标记
- 总RNA分离纯化盒
- PCR产物纯化
- 核酸酶
- 聚合酶
- 反转录酶
