Monitoring the spatial distribution of prespore and pstB cell types is a sensitive method to monitor GSK-3 and Aar activity duringDictyosteliumdevelopment. Cell-specific expression oflacZmarker genes can be readily detected using enzymatic cleavage of the substrate X-gal. This protocol describes a simple method for β-galactosidase staining in developedDictyosteliumstructures.
