Software for:
Making Restriction Maps
Designing PCR primers
Assembling fragments, detecting SNPs, and managing raw sequence data
For:GCG,Mac,PC,UNIX,and on the Web
Restriction Mapping
-Making restriction maps was my first use of “Molecular Biology” software
-Making restriction maps is a routine lab activity that is necessary for any type of cloning project.
-High quality maps are important for publications and exchange of information between researchers or between labs.
Archiving Data
-Maps are a common way for labs to archive information about entire libraries of plasmid constructs.
-It is important that map data are stored in a reliable format so that the obsolescence of a particular computer program does not render archives unusable.
Mapping Software
-Programs vary greatly in sophistication and ease of use
-Simple drawing programs (vector graphics)
-The venerable DNA Strider
-GCG (not a strong point of the package)
-Comprehensive Mac/PC MolBio programs
-Dedicated plasmid drawing programs
-Can it be done on the Web?
-Making high quality graphical restriction maps is one area where Mac/PC programs are much better than GCG or the Web
