Fat tissue provides a rich and easily accessible supply of stem cells that feature the general differentiation potential and plasticity of mesenchymal stem cells. These stem cells, variably designated adipose-derived stem cells (ASCs), hold a great promise for regeneration in vivo and tissue engineering. In order to be able to obtain ASC preparations suitable for basic investigations as well as development of future therapeutic protocols, it is important that the critical isolation steps are properly carried out. Here, we describe in detail enzyme-based procedure for isolation and propagation of ASCs from the human subcutaneous fat tissue that is also adoptable to several animal species. The critical steps and impact of possible modifications on the final outcome are discussed, and useful hints are provided to streamline the troubleshooting.
